Engineering of Bacillus thuringiensis Cry Proteins to Enhance the Activity against Western Corn Rootworm
نویسندگان
چکیده
منابع مشابه
Novel Bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, Diabrotica virgifera virgifera LeConte.
A new family of insecticidal crystal proteins was discovered by screening sporulated Bacillus thuringiensis cultures for oral activity against western corn rootworm (WCR) larvae. B. thuringiensis isolates PS80JJ1, PS149B1, and PS167H2 have WCR insecticidal activity attributable to parasporal inclusion bodies containing proteins with molecular masses of ca. 14 and 44 kDa. The genes encoding thes...
متن کاملBaseline susceptibility of western corn rootworm (Coleoptera: Crysomelidae) to Cry3Bb1 Bacillus thuringiensis toxin.
Susceptibility to Cry3Bb1 toxin from Bacillus thuringiensis (Bt) was determined for western corn rootworm, Diabrotica virgifera virgifera LeConte, neonates from both laboratory and field populations collected from across the Corn Belt. Rootworm larvae were exposed to artificial diet treated with increasing Cry3Bb1 concentrations, and mortality and growth inhibition were evaluated after 4-7 d. T...
متن کاملBaseline Susceptibility of Western Corn Rootworm (Coleoptera: Crysomelidae) to Cry3Bb1 <i>Bacillus thuringiensis</i> Toxin
Susceptibility to Cry3Bb1 toxin from Bacillus thuringiensis (Bt) was determined for western corn rootworm, Diabrotica virgifera virgifera LeConte, neonates from both laboratory and Þeld populations collected from across the Corn Belt. Rootworm larvae were exposed to artiÞcial diet treated with increasing Cry3Bb1 concentrations, and mortality and growth inhibition were evaluated after 4Ð7 d. The...
متن کاملBroad-spectrum resistance to Bacillus thuringiensis toxins by western corn rootworm (Diabrotica virgifera virgifera)
The evolution of resistance and cross-resistance threaten the sustainability of genetically engineered crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a serious pest of maize and has been managed with Bt maize since 2003. We conducted laboratory bioassays with maize hybrids producing...
متن کاملField-evolved resistance by western corn rootworm to multiple Bacillus thuringiensis toxins in transgenic maize.
The widespread planting of crops genetically engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) places intense selective pressure on pest populations to evolve resistance. Western corn rootworm is a key pest of maize, and in continuous maize fields it is often managed through planting of Bt maize. During 2009 and 2010, fields were identified in Iowa...
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ژورنال
عنوان ژورنال: Toxins
سال: 2019
ISSN: 2072-6651
DOI: 10.3390/toxins11030162